Journal: Experimental Dermatology

Article Title: Establishing an allergic eczema model employing recombinant house dust mite allergens Der p 1 and Der p 2 in BALB/c mice

doi: 10.1111/exd.12015

Figure Lengend Snippet: (a) In contrast to PBS-treated mice, animals treated with either rDer p 1 or rDer p 2 showed an increase in epidermal layers and more infiltrates of immune cells into the dermis. (b) The number of mast cells/mm 2 did not differ between treatment groups (n.s.: not significant), the number of eosinophils was significantly elevated in groups treated either with rDer p 1 or rDer p 2 (* P < 0.05; ** P < 0.001). (c) Immunhistochemical staining for CD3 was performed on deparaffinized skin biopsy sections of mice. After primary CD3-specific antibody, goat anti-rat AF568 antibody was used. Finally, slides were mounted with fluoromount and analysed by light microscope; quantification was calculated using TissueQuest cell analysis software from TissueGnostics. The two groups, treated with rDer p 1 or rDer p 2 showed enhanced invasion of CD3 + T cells into the epidermis, compared with control.

Article Snippet: After final washing steps, slides were mounted with fluoromount and analysed by light microscope and quantification was performed using TissueQuest® cell analysis software from TissueGnostics, Vienna, Austria.

Techniques: Staining, Light Microscopy, Cell Analysis, Software, Control

Journal: The Journal of Biological Chemistry

Article Title: Blockade of Fas Signaling in Breast Cancer Cells Suppresses Tumor Growth and Metastasis via Disruption of Fas Signaling-initiated Cancer-related Inflammation *

doi: 10.1074/jbc.M113.525014

Figure Lengend Snippet: Higher expression of Fas in human breast cancer tissue correlates with poorer prognosis in breast cancer patients. A and B, protein expression of Fas in human breast cancer tissue derived from Cohort 1 and Cohort 2 was detected by IHC. One representative image for lower or higher expression of Fas is shown. The bar represents 100 μm (A, left panel). The mean intensities of Fas in tumors was measured by defining regions of interest using automated cell acquisition and quantification software for IHC (Histoquest) in a FACS-like manner of scattergram analysis (A, right panel). Higher levels of Fas in human breast cancer tissues were correlated with reduced overall survival in breast cancer patients. Kaplan-Meier survival curves of overall survival in Cohort 1 (B) survival data were analyzed by log-rank statistics, and the p value is indicated.

Article Snippet: Staining intensity was quantified by tissue cytometry using the HistoQuest analysis software (TissueGnostics) as previously described ( 39 ).

Techniques: Expressing, Derivative Assay, Software